I'm using Ardour 5.3 with Fedora 24. I've been able to record with both a Focursite Scarlett 18i8, and with a Focusrite Saffire Pro26 (using ffado 2٫2, though it doesn't officially support the Pro26). So far, all great.
However, I can't get any playback via either audio interface when selected in QjackCTL. If I select the internal interface, it plays fine on my internal speakers. So - as far as I can tell, the problem is between JACK and the interfaces.
When changing interfaces, all I've adjusted is 'driver' and 'interface' in the Parameters tab and 'input device' and 'output device' in the advanced tab.
I can post any diagnostic output or further info, but I'm not sure what is relevant. My Googling hasn't turned up anything useful yet, so any help at all would be very gratefully received!